Fig. 2.

MASTL depletion induces cell death in breast cancer cells. The cells were transfected with either the control (Ctrl) siRNA or MASTL siRNAs, as indicated, for 48 h. a Representative light microscopy images of control (Ctrl) or MASTL.1 siRNA-depleted cells. Scale bar = 100 μm. b The cells were analyzed by immunoblotting or c immunofluorescence analysis with the indicated antibodies. Scale bars = 10 μm. d MCF7, T47D, HCC1937, and SKBR3 cells treated with either control (Ctrl) siRNA or MASTL.5 siRNA were analyzed by immunoblotting with the indicated antibodies. e The trypan blue exclusion assay was used to assess the effect of the cells treated with either control (Ctrl) siRNA, MASTL.4 siRNA, or MASTL.5 siRNA. f MCF7 cells were transfected with either control (Ctrl) siRNA, MASTL.5 siRNA, or both MASTL.5 siRNA and mouse MASTL vector for 48 h and then analyzed by immunoblotting for the indicated antibodies. The data represent typical results and are presented as the mean ± standard deviation of three independent experiments; **P < 0.01 and *P < 0.05