Table 3.
Growth profile of Pseudomonas putida KT2440 wt and Δalr strains on 19 d- or l-AAs as a sole source of C and N.
wt | Δalr | wt | Δalr | |
---|---|---|---|---|
C and N source, d-AAs | C and N source, l-AAs | |||
Ala | +++ | +++ | +++ | +++ |
Arg | ++ | ++ | +++ | + |
Asn | – | – | +++ | +++ |
Asp | – | – | +++ | +++ |
Cys | – | – | – | – |
Glu | – | – | +++ | +++ |
Gln | – | – | +++ | +++ |
His | – | – | +++ | +++ |
Ile | – | – | ++ | ++ |
Leu | – | – | – | – |
Lys | ++ | ++ | ++ | + |
Met | – | – | – | – |
Phe | + | + | + | + |
Pro | – | – | +++ | +++ |
Ser | – | – | ++ | ++ |
Thr | – | – | – | – |
Trp | – | – | – | – |
Tyr | – | – | – | – |
Val | – | – | ++ | ++ |
+++, abs at 600 nm ≥ 0.5 after 24 h of incubation; ++, abs at 600 nm ≥ 0.5 after 48 h of incubation; +, abs at 600 nm ≥ 0.5 after 72 h of incubation; –, abs at 600 nm < 0.5 after 72 h of incubation; Growth assays were performed in triplicate. The shading highlights the only significant differences (p < 0.05, Student's t-test) between the two strains.