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. 2018 Jul 5;11(4):1044–1052. doi: 10.1016/j.tranon.2018.06.008

Figure 1.

Figure 1

Radiation sensitivity in two colon cancer cell lines of HCT-116 and HT-29. (A) Cells were irradiated with 0, 5, and 10 Gy and then stained with Annexin V and PI for flow cytometry. Numbers represent the percent of cells in the fraction. (B) The Annexin V/PI-positive cells were calculated as the percentage. Columns, mean; error bars, SEM, from three independent experiments. *P < .05. (C) Cells were irradiated with 0, 5, and 10 Gy and then stained with PSP1 for FACS. Apoptosis was determined by FACS by using PSP1 in HT29 and HCT116 cells at 48 hours after radiation (0, 5, and 10 Gy). (D) The PSP1-positive cells were calculated as the percentage. Columns, mean; error bars, SEM, from three independent experiments. *P < .05.