Figure 1.

Schematic of the New Bac System and Verification for rAAV Production upon Single BEV/Cap-(ITR-GFP)-Rep Infection of Sf9 Cells

(A) Schematic of pFD/Cap-(ITR-GFP)-Rep construct and rAAV production upon BEV/Cap-(ITR-GFP)-Rep infection of Sf9 cells. The functional elements are represented by open boxes and separated by vertical lines. Promoters are represented by arrows. (B) Fluorescence microscopy observation of GFP expression in BEV/Cap2-(ITR-GFP)-Rep-infected Sf9 Cells. (C) Fluorescence microscopy observation of 96-well plate cultured HEK293 and Sf9 cells infected with HEK293 cell-derived AAV2 (293) at an MOI of 5,000, supernatants of BEV/Cap2-(ITR-GFP)-Rep-infected Sf9 cells (10 μL culture supernatants per well), and supernatants of BEV/Cap2-(ITR-GFP)-Rep-infected Sf9 cells lysate samples (10 μL lysate supernatants of 1 × 10⁴ cells Sf9 cells per well), respectively. Treated, heat treatment with 60°C for 30 min; p.i., post-infection. Representative fields are shown; upper images are green fluorescence fields, lower images are bright fields.