Figure 3.

Analysis of the BEV Stability upon Serial Passages

(A) Western blot analysis of Rep and Cap protein expression. Passage number is indicated above each lane. The AAV Rep and Cap proteins and endogenous cellular protein β-tubulin are indicated in the left margin. (B) Analysis of the yields of rAAV2 in Sf9 cells upon infection with serial passages of BEV/Cap2-(ITR-GFP)-Rep stocks from passage (P)1 to P8. The yields of rAAVs were determined as vector genomes per cell (VG/cell). All experiments were done in triplicate. Mean ± SD values are presented. Asterisks depict Tukey’s multiple comparison test significance between groups following ANOVA, *p < 0.05 and **p < 0.01; ns, no significant differences.