Figure 6.

Analysis of rAAV Produced in Beet Armyworm Larvae upon the New BEV/Cap2-(ITR-GFP)-Rep Infection

(A) Fluorescence microscopy observation of HEK293 and Sf9 cells infected with the supernatant of infected or uninfected larvae lysate samples. Treated, heat treatment with 60°C for 30 min; p.i., post-infection. Representative fields are shown; upper images are green fluorescence fields, lower images are bright fields. (B) Comparison of transduction efficiency among rAAV2 derived from HEK293 cells, Sf9 cells, and larvae. HEK293 cells were infected with gradient diluents of rAAVs and examined under a fluorescence microscope 2 days post-infection. (C) Transmission electron microscopy analysis of negatively stained purified rAAV2. Original magnification ×100,000. Scale bars, 100 nm. (D) Transduction activity analysis of larvae-derived rAAV2 in mouse brain DG. Scale bar, 500 μm. Representative fields are shown. (E) Analysis of the yields of rAAV2 in different parts of the larvae. All experiments were done in triplicate and displayed as mean ± SD.