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. 2018 Jul 4;20(8):800–812. doi: 10.1016/j.neo.2018.05.008

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Expression of EMT markers in mixed-cell spheroids and in vivo tissues. (A) The expression of the EMT markers E-cadherin and vimentin was detected by immunofluorescence staining of paraffin sections of LX-2 and Huh-7 monospheroids and mixed-cell spheroids. Counterstain, DAPI (blue). Scale bars, 100 μm. Relative intensity across sections was expressed as mean ± SE of more than five replicates. **P < .005 and ***P < .001 relative to Huh-7 monospheroids. (B) Comparison of EMT marker expression in Huh-7/LX-2 mixed-cell spheroids with that in paraffin sections of human HCC and adjacent nontumor (NT), and HCC xenograft tissue samples. All the antibodies show specificity against human and mouse antigen. Scale bars, 100 μm (inset) and 50 μm. T, tumor region; S, stroma region; arrows, expression in patches.