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. 2018 Jul 4;20(8):800–812. doi: 10.1016/j.neo.2018.05.008

Figure 6.

Figure 6

Invasive migratory profile of mixed-cell spheroids. (A) Cell invasion and migration from monospheroids embedded in Matrigel for 72 hours. Scale bar, 100 μm. (B) In mixed-cell spheroids, small cellular protrusions with invadosomes were visible after 24 hours that continuously extended into the matrix over 72 hours. The relative invasion area was quantified using ImageJ software. Scale bars, 100 μm. *P < .05 and **P < .005. (C) Fluorescence dye tracing of Huh-7 cells (PKH26: red) to show migration from mixed-cell spheroids. Red arrows, invadosome; white arrows, PKH26-labeled Huh-7 cells; yellow arrows, DAPI-stained (blue) LX-2 cells; dotted line, spheroid margin. Scale bar, 50 μm.