Figure 1. Effects of in ovo exposure to fulvestrant and 17α-ethinylestradiol on mortality, left and right gonad surface area, cortex thickness and percentage of seminiferous tubules of left gonad of embryos of the domestic fowl (Gallus g. domesticus).

Effects of in ovo exposure to fulvestrant (FUL, 0.1, 1, 10 µg/g egg) and 17α-ethinylestradiol (EE₂; 20 ng/g egg) on mortality (A), left and right gonad surface area (B) and cortex thickness and percentage of seminiferous tubules of left gonad (C) of embryos of the domestic fowl (Gallus g. domesticus) on embryonic day 19. Statistical analysis by Fisher’s exact test (A) and one-way ANOVA with Dunnett’s multiple comparison test (B, C). Grey background distinguishes the co-exposure to fulvestrant and EE₂. Lowercase indicate significant differences compared to the solvent control. Level of significance: a: p < 0.05; b: p < 0.01; c: p < 0.001.