Table 1.
Overview of characteristics of representative broadly neutralizing antibodiesa
| Sequence ID | Chain | V gene and allele | D gene and allele | J gene and allele | CDR1 length | CDR2 length | CDR3 length | SHM, % nt | Ref. |
|---|---|---|---|---|---|---|---|---|---|
| First generation | |||||||||
| 2G12 | Heavy | VH3-21*01 | D6-6*01 | JH3*01 | 8 | 8 | 16 | 20.3 | 55,56 |
| Light | VK1-5*03 | JK1*01 | 6 | 3 | 9 | 12.2 | |||
| b12 | Heavy | VH1-3*01 | D2-21*01 | JH6*03 | 8 | 8 | 20 | 13.8 | 50,51 |
| Light | VK3-20*01 | JK2*01 | 7 | 3 | 9 | 13.4 | |||
| 2F5 | Heavy | VH2-5*02 | D3-3*01 | JH6*02 | 10 | 7 | 24 | 12.1 | 53,54,56 |
| Light | VK1D-13*02 | JK4*01 | 6 | 3 | 9 | 11.8 | |||
| 4E10 | Heavy | VH1-69*06 | D6-19*06 | JH4*02 | 8 | 8 | 20 | 6.8 | 54a,56 |
| Light | VK3-20*01 | JK1*01 | 7 | 3 | 9 | 4.7 | |||
| Second generation | |||||||||
| PG9 | Heavy | VH3-33*05 | D3-3*01 | JH6*03 | 8 | 8 | 30 | 12.0 | 74 |
| Light | VL2-14*01 | JL3*02 | 9 | 3 | 10 | 9.2 | |||
| PG16 | Heavy | VH3-33*05 | D3-3*01 | JH6*03 | 8 | 8 | 30 | 13.1 | 74 |
| Light | VL2-14*01 | JL3*01 | 9 | 3 | 10 | 12.2 | |||
| PGT145 | Heavy | VH1-8*01 | D4-17*01 | JH6*02 | 8 | 8 | 33 | 16.2 | 75 |
| Light | VK2D-28*01 | JK1*01 | 11 | 3 | 9 | 15.5 | |||
| CAP256-VRC26.09 | Heavy | VH3-30*18 | D3-3*01 | JH3*02 | 8 | 8 | 39 | 12.8 | 77 |
| Light | VL1-51*02 | JL1*01 | 8 | 3 | 12 | 9.7 | |||
| PGDM1400 | Heavy | VH1-8*01 | D4-17*01 | JH6*02 | 8 | 8 | 34 | 24.3 | 81 |
| Light | VK2D-28*01 | JK1*01 | 11 | 3 | 9 | 11.3 | |||
| PGT121 | Heavy | VH4-59*07 | D3-3*02 | JH6*03 | 8 | 7 | 26 | 17.8 | 75 |
| Light | VL3-21*03 | JL3*02 | 6 | 3 | 12 | 15.5 | |||
| PGT124 | Heavy | VH4-59*03 | D3-3*01 | JH6*03 | 8 | 7 | 26 | 15.0 | 75 |
| Light | VL3-21*01 | JL3*02 | 6 | 3 | 12 | 16.6 | |||
| PGT128 | Heavy | VH4-39*07 | D3-3*01 | JH5*02 | 10 | 13 | 21 | 19.0 | 75 |
| Light | VL2-8*01 | JL3*01 | 4 | 3 | 10 | 8.7 | |||
| PGT135 | Heavy | VH4-39*07 | D2-21*01 | JH5*02 | 15 | 7 | 20 | 17.1 | 75 |
| Light | VK3-15*01 | JK1*01 | 6 | 3 | 9 | 15.9 | |||
| 10-1074 | Heavy | VH4-4*08 | D3-3*02 | JH6*03 | 8 | 7 | 26 | 26.7 | 83 |
| Light | VL3-9*02 | JL3*02 | 6 | 3 | 12 | 30.1 | |||
| VRC01 | Heavy | VH1-2*02 | D5-18*01 | JH2*01 | 8 | 8 | 14 | 29.7 | 79 |
| Light | VK3-NL1*01 | JK2*04 | 4 | 3 | 5 | 16.6 | |||
| 3BNC117 | Heavy | VH1-2*02 | D6-25*01 | JH2*01 | 8 | 8 | 12 | 23.5 | 158 |
| Light | VK1D-33*01 | JK3*01 | 6 | 3 | 5 | 16.6 | |||
| CH103 | Heavy | VH4-59*01 | D3-9*01 | JH4*02 | 8 | 7 | 15 | 18.0 | 132 |
| Light | VL3-1*01 | JL1*01 | 6 | 3 | 10 | 10.7 | |||
| PGT151 | Heavy | VH3-30-3*03 | D3-3*01 | JH6*02 | 8 | 8 | 28 | 18.5 | 85,98 |
| Light | VK2D-29*02 | JK4*02 | 11 | 3 | 9 | 12.6 | |||
| 35O22 | Heavy | VH1-18*03 | D3-10*01 | JH5*02 | 8 | 8 | 16 | 21.9 | 84 |
| Light | VL2-23*03 | JL1*01 | 9 | 3 | 10 | 22.4 | |||
| 8ANC195 | Heavy | VH1-69D*01 | D3-3-01 | JH4*02 | 9 | 6 | 22 | 27.2 | 86,87 |
| Light | VK1-5*03 | JK1*01 | 7 | 3 | 9 | 14.9 | |||
| 10E8 | Heavy | VH3-15*05 | D3-3*01 | JH1*01 | 8 | 10 | 22 | 19.4 | 78 |
| Light | VL3-19*01 | JL3*02 | 6 | 3 | 12 | 14.2 | |||
Abbreviation: SHM, somatic hypermutation.
V(D)J assignment was performed using the AbStar software (B. Briney et al., personal communication). The total number of somatic hypermutations corresponds to the sum of the mutations found in V and J elements. The epitope specificity is indicated by color: yellow for MPER (membrane-proximal external region), red for gp120-gp41 interface, purple for high-mannose patch, blue for apex, and green for CD4 binding site. First-generation bnAb sequences were obtained from personal communications; second-generation bnAb sequences were obtained from GenBank. Note that the short length of the D region often prevents an unequivocal identification of the encoding gene allele.