(A) Two representative mice imaged by PET/CT using a 64Cu-labeled
anti-PD-L1 antibody are shown. BALB/c mice had received either
JAK2V617F-transfected (right) or WT syngeneic BM (left) after total body
irradiation (TBI, 6.5 Gy). The signal intensity indicates areas with high PD-L1
expression. Arrows point towards the spleen (high signal intensity). The image is
representative of 4 mice per group with comparable signal patterns.
(B) The bar diagram shows the signal intensity in the indicated organs of
mice transplanted as described in (A). Data are pooled from 4 or 5 mice per
group.
(C, D) The bar diagram shows the MFI for PD-L1 on different cells and
platelets in the BM (C) or spleen (D) of mice transplanted as
described in (A). n=5 each group.
(E, F) The bar diagram shows the MFI for PD-L1 on different cells and
platelets in BM (E) and spleen (F) of JAK2-FLEX/Mx-Cre mice
(n=3 each group).
(G) The bar diagram shows the JAK2V617F allele burden in
different cell populations isolated by cell sorting from 15 MPN patients. The
JAK2V617F allele burden was determined by qPCR (n=15 each group).
(H) The Western blot shows the amounts of pSTAT3 and STAT3 in different cell
populations isolated by cell sorting from a representative polycythemia vera patient.
(I) The bar diagram shows the ratios of pSTAT3/STAT3/β-actin in
different cell populations isolated by cell sorting from multiple MPN patients
(n=5 for CD42b, CD19, CD15, and CD3, n=3 for CD14).