Table 2.
Primer Sequences Used for Real-time Polymerase Chain Reaction Analysis
| Genes | Sequence (5’–3’) | Amplicon |
|---|---|---|
| GAD2 | FP: CGAGTCCCTGGAGCAGATCCTGGTT RP: GTCAGCCATTCTCCAGCTAGGCCAATAATA |
132 bp |
| GABA-T | FP: GACGGAAGTCCCAGGGCC RP: GGAGCCACCGAGAGCAA |
315 bp |
| π subunit of GABA-AR | FP: ACGTCGAGGTCGGCAGAA RP: CCCGCCTCGTGGAGTTC |
275 bp |
| B1 subunit of GABA-BR | FP:CAGATAAATGGATTGGAGGGT RP:GAGAACTGAGACGGAGATAAAGAG |
101 bp |
| B2 subunit of GABA-BR | FP:GAGTCCACGCCATCTTCAAAAAT RP:TCAGGATACACAGGTCGATCAGC |
108 bp |
| GAT-2 | FP: CCCCAGGTGTGGATGGATGC RP: TGCTCCTGAGACATGAAGCCCA |
203 bp |
| IL-1β | FP: CTCTCTCACCTCTCCTACTCAC RP: ACACTGCTACTTCTTGCCCC |
188 bp |
| IL-6 | FP: GTAGCCGCCCCACACAGA RP: CATGTCTCCTTTCTCAGGGCTG |
101 bp |
| TNF-α | FP: CCCCAGGGACCTCTCTCTAA RP: TGAGGTACAGGCCCTCTGAT |
212 bp |
| IL-8 | FP: ACTGAGAGTGATTGAGAGTGGAC RP: AACCCTCTGCACCCAGTTTTC |
112 bp |
| GAPDH | FP: GCTCCTCCTGTTCGACAGTCA RP: GCAACAATATCCACTTTACCAG |
180 bp |
GAD2, glutamic acid decarboxylase 2; GABA-T, γ-aminobutyric acid transaminase; GABA-AR, GABA-A receptor; GABA-BR, GABA-B receptor; GAT-2, GABA transporter subtype 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; FP, forward primer; RP, reverse primer.