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Comparison of acetylcholine (ACh)-induced tension of colonic longitudinal smooth muscle strips (CLSMs) after methylene blue (inhibitor of guanylate cyclase) and L-NAME (inhibitor of nitric oxide synthase) (means ± SE, n = 6). (A) Pretreated with methylene blue. (B) Pretreated with L-NAME. NH, normal control (no handling); MMS, neonatal maternal separation; RS, restraint stress; FLCWK, Fengliao-Changweikang (2.25 mg/100 g).
