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. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: Sci Transl Med. 2017 Sep 20;9(408):eaan4220. doi: 10.1126/scitranslmed.aan4220

Fig. 2. PVSRIPO oncolysis releases tumor antigens, dsRNA, and DAMPs.

Fig. 2.

DM6 and MDA-MB231 cells were infected with PVSRIPO. (A) Silver stain was performed on oncolysates/corresponding supernatants. (B) Immunoblots from oncolysates/corresponding supernatants for tumor antigens (MART1/CEA), DAMPs (HSPs, HMGB1), HSP60 (mitochondrial), Na+/K+ ATPase (membrane-associated), PARP (nucleus), and tubulin (cytoplasm). (C) Immunoblot for dsRNA was performed from oncolysates/ corresponding supernatants of DM6 and MDA-MB231 cells (48 hpi); Ponceau S stain serves as loading control. Loss of total protein late during PVSRIPO infection corresponds with cell lysis. All experiments were repeated twice, and a representative series is shown.