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. Author manuscript; available in PMC: 2018 Jul 6.
Published in final edited form as: Cell Rep. 2018 Jun 12;23(11):3146–3151. doi: 10.1016/j.celrep.2018.05.052

Figure 4. Deletion of Zebrafish tbx5a cns12sh DNA Element.

Figure 4

(A) CRISPR/Cas9 gene editing strategy to delete the zebrafishtbx5a cns12shelement (Adachi et al., 2016). (B) Zebrafish 5-day-old embryos showing pectoral fins (arrows) in wild-type as well as heterozygous and homozygous cns12sh deletion mutants; scale bar: 1 mm (same for all panels).

(C) Comparison of pectoral fin lengths; data are expressed as mean ± SD. For all comparisons, p > 0.05 (not significant difference) using one-way ANOVA non-parametric test (n = 4 biological replicates for each genotype, stage-matched).

See also Figure S3.