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. 2018 Jun 22;9(48):28849–28865. doi: 10.18632/oncotarget.25599

Figure 4. Direct regulation of ITGA3 by miR-124-3p in PDAC cell lines.

Figure 4

(A) Expression levels of ITGA3 in PDAC clinical specimens and cell lines were determined by qRT-PCR. Data were normalized to GUSB expression. (B) Expression levels of ITGA3 and miR-124-3p were negatively correlated. (C) ITGA3 mRNA expression in PDAC cell lines was evaluated by qRT-PCR 72 h after transfection with miR-124-3p. GUSB was used as an internal control. *, P < 0.0001. (D) ITGA3 protein expression in PDAC cell lines was evaluated by western blot analysis 96 h after transfection with miR-124-3p. GAPDH was used as a loading control. (E) miR-124-3p binding sites in the 3′-UTR of ITGA3 mRNA. Dual luciferase reporter assays using vectors encoding the putative miR-124-3p (positions 1160-1166) target site of the ITGA3 3′-UTR for both wild-type and deleted regions. Normalized data were calculated as ratios of Renilla/firefly luciferase activities. *, P < 0.0001.