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. 2018 Jun 22;9(48):28849–28865. doi: 10.18632/oncotarget.25599

Figure 6. ITGA3 mRNA and protein expression after si-ITGA3 transfection and effects of ITGA3 silencing in PDAC cell lines.

Figure 6

(A) ITGA3 mRNA expression in PDAC cell lines was evaluated by qRT-PCR 72 h after transfection with si-ITGA3-1 or si-ITGA3-2. GUSB was used as an internal control. (B) ITGA3 protein expression in PDAC cell lines was evaluated by western blot analysis 96 h after transfection with si-ITGA3-1 and si-ITGA3-2. GAPDH was used as a loading control. (C) Cell proliferation was determined using XTT assays 72 h after transfection with 10 nM si-ITGA3-1 or si-ITGA3-2. *, P < 0.0001. (D) Cell migration activity was determined using migration assays. *, P < 0.0001. (E) Cell invasion activity was determined by Matrigel invasion assays. *, P < 0.0001.