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. 2018 Jun 22;9(48):28849–28865. doi: 10.18632/oncotarget.25599

Figure 7. ITGB1 mRNA and protein expression after si-ITGB1 transfection and effects of ITGB1 silencing in PDAC cell lines.

Figure 7

(A) ITGB1 mRNA expression in PDAC cell lines was evaluated by qRT-PCR 72 h after transfection with si-ITGB1-1 or si-ITGB1-2. GUSB was used as an internal control. (B) ITGB1 protein expression in PDAC cell lines was evaluated by western blot analysis 96 h after transfection with si-ITGB1-1 and si-ITGB1-2. GAPDH was used as a loading control. (C) Cell proliferation was determined with the XTT assays 72 h after transfection with 10 nM si-ITGB1-1 or si-ITGB1-2. *, P < 0.0001. (D) Cell migration activity was determined using migration assays. *, P < 0.0001. (E) Cell invasion activity was determined by Matrigel invasion assays. *, P < 0.0001.