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. 2018 Jun 22;9(48):28877–28896. doi: 10.18632/oncotarget.25608

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Copyright: © 2018 Hensel et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Immunohistochemical or immunofluorescent detection of endothelial cell markers (Endomucin (A) and Cd105 (Endoglin) (B)), artery markers (αSMA (C) and Elastin (D)), pericyte markers (Cspg4/Ng2 (E), Pdgfrb (F) and Sca1 (G)) and neuronal/neurite markers (β3 tubulin (H) and Gap43 (I) in control bones, MDA-MB231 xenografts, PC-3 xenografts and VCaP xenografts. (J) Immunofluorescent detection of Gap43 positive cells in proximity to Elastin positive cells in PC-3 and MDA-MB231 xenografts. Scale bar = 50 μm. (K) Quantification of percent positive staining area per field of view (FOV) for endomucin, Cd105 (Endoglin), αSMA, Alexa Fluor 633 (=Elastin), Cspg4/Ng2, Pdgfrb, Sca1, β3 tubulin and Gap43.