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Immunohistochemical detection of αSMA (A-C) and β3 tubulin (E-G) in normal bone (A, E), in osteolytic bone metastasis (B, F) and in osteoblastic bone metastasis (C, G). Scale bar = 50 μm. Positive staining area of αSMA (D) and β3 tubulin (H) was quantified in 2 specimens of osteoblastic bone metastasis and 3 specimens of osteolytic bone metastasis. Dotted line in panel C highlights the bone surface.
