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. 2018 Jul 6;13(7):e0199974. doi: 10.1371/journal.pone.0199974

Table 1. List of primers and PCR conditions used in this study.

Target gene Primers Sequence (5´- 3´) PCR Conditions Product (bp) Reference
16S MD16S1 ATCTAATGGCTTAACCATTAAAC 95°C/10 min, 35 cycles: 95°C/30s, 59°C/90s, 72°C/1 min, and 72°C/10 min. 857 for Campylobacter genus identification. (18, 19)
rRNA MD16S2 GGACGGTAACTAGTTTAGTATT
mapA MDmapA1 CTATTTTATTTTTGAGTGCTTGTG 589 for C. jejuni species identification. (18)
MDmapA2 GCTTTATTTGCCATTTGTTTTATTA
ceuE col3 AATTGAAAATTGCTCCAACTATG 462 for C. coli species identification. (18)
MDcol2 TGATTTTATTATTTGTAGCAGCG
PCR-RFLP (gyrA) C. jejuni cjgyrAM1 AAATCAGCCCGTATAGTGGGTGCTGTTATAGGTCGTTATCACCCACACATGGAGGT 94°C/5 min, 30 cycles: 94°C/1 min, 51°C/1 min,72°C/45s, and 72°C/7 min. 179 detection gyrA (C. jejuni). (15, 22)
cjgyrA2 TCAGTATAACGCATCGCAGC
PCR-RFLP (gyrA) C. coli colgyrA AAATCTGCTCGTATAGTAGGGGATGTTATCGGTAAGTATCATCCACATGGCGGT 94°C/5 min, 30 cycles: 94°C/1 min, 55°C/1 min,72°C/45s, and 72°C/7 min. 179 detection gyrA (C. coli). (13, 15)
colgyrA2 TCAGTATAACGCATCGCAGC