16S |
MD16S1 |
ATCTAATGGCTTAACCATTAAAC |
95°C/10 min, 35 cycles: 95°C/30s, 59°C/90s, 72°C/1 min, and 72°C/10 min. |
857 for Campylobacter genus identification. |
(18, 19) |
rRNA |
MD16S2 |
GGACGGTAACTAGTTTAGTATT |
mapA |
MDmapA1 |
CTATTTTATTTTTGAGTGCTTGTG |
589 for C. jejuni species identification. |
(18) |
MDmapA2 |
GCTTTATTTGCCATTTGTTTTATTA |
ceuE |
col3 |
AATTGAAAATTGCTCCAACTATG |
462 for C. coli species identification. |
(18) |
MDcol2 |
TGATTTTATTATTTGTAGCAGCG |
PCR-RFLP (gyrA) C. jejuni
|
cjgyrAM1 |
AAATCAGCCCGTATAGTGGGTGCTGTTATAGGTCGTTATCACCCACACATGGAGGT |
94°C/5 min, 30 cycles: 94°C/1 min, 51°C/1 min,72°C/45s, and 72°C/7 min. |
179 detection gyrA (C. jejuni). |
(15, 22) |
cjgyrA2 |
TCAGTATAACGCATCGCAGC |
PCR-RFLP (gyrA) C. coli
|
colgyrA |
AAATCTGCTCGTATAGTAGGGGATGTTATCGGTAAGTATCATCCACATGGCGGT |
94°C/5 min, 30 cycles: 94°C/1 min, 55°C/1 min,72°C/45s, and 72°C/7 min. |
179 detection gyrA (C. coli). |
(13, 15) |
colgyrA2 |
TCAGTATAACGCATCGCAGC |