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. 2018 Jul 6;13(7):e0200210. doi: 10.1371/journal.pone.0200210

Fig 1. Overexpression of the GPA genes in hFIB cultures.

Fig 1

(A) Significantly increased mRNA expression of the genes was observed compared to non-infected control cultures by qPCR analyses. Asterisks: p-value<0,05. (B) GPA protein expression in hFIB cultures simultaneously transduced with pSIN-EF2-Pou4f3-Pur, pSIN-EF2-GFI1-Pur and pLV-ATOH1-IRES-eGFP lentiviruses. Immunostaining of GPA hFIB cultures indicated that a high rate of the GFP(+) cells also expressed the Pou4f3 and the GFI1 proteins. Scale bar: 50μm.