Table 2. Concentrations, areas under the curves, and best cutoff values of MIPs, MCP, and cytokines in amniotic fluid, cervicovaginal fluid, and maternal blood in relation to positive amniotic fluid culture resultsa.
| Amniotic fluid culture | P-valueb | AUCc | Cutoff value |
<LLOQ | ||
|---|---|---|---|---|---|---|
| Negative (n = 43) | Positive (n = 32) | |||||
| Amniotic fluid (AF) | ||||||
| AF IL-6 | 408.4 (28.5–45372.9) | 9932.7 (79.1–157349.5) | < 0.001 | 0.857 | 1488.7 | 0% |
| AF IL-8 | 256.7 (48.3–16428.9) | 5402.2 (51.4–152880.8) | < 0.001 | 0.852 | 988.46 | 0% |
| AF MCP-1 | 237.8 (10.4–15643.5) | 4308.8 (36.9–21194.7) | < 0.001 | 0.836 | 1166.78 | 4% |
| AF MIP-1α | 12.0 (12.0–1048.8) | 266.1 (12.0–11341.0) | < 0.001 | 0.877 | 12.7 | 52% |
| AF MIP-1β | 34.7 (8.5–7529.7) | 2647.8 (8.5–23196.9) | < 0.001 | 0.872 | 310.65 | 27% |
| Percentage above LLOQ for AF MIP-1α | 18.6% (8) | 87.5% (28) | < 0.001 | |||
| Percentage above LLOQ for AF MIP-1β | 58.1% (25) | 93.8% (30) | 0.001 | |||
| Cervicovaginal fluid (CVF) | ||||||
| CVF IL-6 | 174.5 (15.6–1445.7) | 656.9 (15.6–6473.4) | 0.001 | 0.732 | 310.79 | 3% |
| CVF IL-8 | 1451.4 (54.0–25318.7) | 6459.1 (778.0–28766.4) | < 0.001 | 0.765 | 2281.14 | 0% |
| CVF MCP-1 | 446.8 (51.1–7264.9) | 1475.3 (142.5–7195.2) | 0.001 | 0.721 | 1341.67 | 0% |
| CVF MIP-1α | 49.1 (12.1–949.3) | 185.3 (12.1–3365.4) | <0.001 | 0.745 | 124.16 | 7% |
| CVF MIP-1β | 495.4 (28.2–6583.2) | 1480.5 (31.6–7609.3) | < 0.001 | 0.765 | 990.57 | 0% |
| Maternal blood (MB) | ||||||
| MB IL-6 | 9.2 (9.2–38.0) | 9.2 (9.2–34.4) | 0.004 | 0.632 | 9.16 | 81% |
| MB IL-8 | 10.0 (10.0–77.7) | 10.0 (10.0–370.2) | 0.799 | 0.512 | 11.56 | 78% |
| MB MCP-1 | 31.1 (9.8–137.4) | 32.3 (9.8–242.7) | 0.932 | 0.506 | 25.06 | 0% |
| MB MIP-1α | 1.0 (0.2–20.4) | 0.8 (0.2–9.3) | 0.265 | 0.425 | 0.77 | 3% |
| MB MIP-1β | 78.6 (24.5–160.1) | 96.4 (20.1–859.1) | 0.120 | 0.605 | 160.1 | 0% |
| Percentage above LLOQ for MB IL-6 | 7.0% (3) | 28.1% (9) | 0.023 | |||
| Percentage above LLOQ for MB IL-8 | 20.9% (9) | 21.9% (7) | 0.921 | |||
Data are given as the median (range) (pg/mL).
AUC, areas under the curves; LLOQ, lower limit of quantification; AF, amniotic fluid; CVF, cervicovaginal fluid; MB, maternal blood; IL, interleukin; MCP-1, monocyte chemotactic protein-1; MIP, macrophage inflammatory protein.
aSignificant findings (p < 0.01) after Bonferroni correction for multiple testing (α threshold = 0.05/5) are presented in bold letters.
bMann-Whitney U test.
cReceiver operating characteristic curve analysis.