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. 2018 May 17;42(2):919–925. doi: 10.3892/ijmm.2018.3687

Figure 4.

Figure 4

Neuronal nitric oxide synthase (nNOS) protein levels in dorsal root ganglion (DRG) neurons under tumor necrosis factor-α (TNF-α) treatment with or without p38 MAPK knockdown. (A) DRG neurons were transfected with p38 MAPK (p38)-siRNA (lane 3), using untreated cells (NC; lane 1) and cells transfected with scramble control siRNA (Scr; lane 2) as controls. The protein levels of p38 MAPK were determined with western blot analyses. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Density of the p38 MAPK blot was normalized against that of the GAPDH blot to obtain a relative blot density, which was expressed as fold changes to that of NC (designated as 1). *p<0.05 vs. NC. (B) DRG neurons were treated with TNF-α (40 ng/ml) for 1 (lane 2), 5 (lane 3), 10 (lane 4), 15 (lane 5), 20 (lane 6) and 25 (lane 7) hours with or without transfection of scramble control siRNA (Scr; lane 8) or p38 MAPK (p38)-siRNA (lane 9). Untreated cells (NC; lane 1) were used as a control. The protein levels of nNOS were determined with western blot analyses. GAPDH was used as a loading control. Density of the nNOS blot was normalized against that of the GAPDH blot to obtain a relative blot density, which was expressed as fold changes to that of NC (designated as 1). *p<0.05 vs. NC; #p<0.05 vs. immediate previous experimental group.