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. 2018 May 17;42(2):919–925. doi: 10.3892/ijmm.2018.3687

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Copyright: © Tan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Neuronal nitric oxide synthase (nNOS) mRNA levels in dorsal root ganglion (DRG) neurons under tumor necrosis factor-α (TNF-α) treatment in the presence of synthetic cannabinoid WIN-55,212-2 mesylate (WIN-55). DRG neurons were treated with TNF-α (40 ng/ml) in the presence of WIN-55 (100, 200, 300 and 400 ng/ml) for 1, 5, 10, 15, 20 and 25 h. Cells treated with TNF-α only were used as a control. The nNOS mRNA levels was measured and expressed as fold-changes to that of untreated cells (designated as 1). ^*p<0.05 vs. control; ^{^}p<0.05 vs. immediate lower concentration; ^#p<0.05 vs. immediate previous time-point.