Skip to main content
. 2018 May 17;42(2):919–925. doi: 10.3892/ijmm.2018.3687

Figure 7.

Figure 7

Neuronal nitric oxide synthase (nNOS) mRNA stability in dorsal root ganglion (DRG) neurons under tumor necrosis factor-α (TNF-α) treatment in the presence of synthetic cannabinoid WIN-55,212-2 mesylate (WIN-55) or p38 MAPK knockdown. DRG neurons with or without transfection of p38 MAPK (p38)-siRNA or scramble control siRNA (Scr) were treated with TNF-α (5, 20 and 40 ng/ml) for 25 h in the presence of WIN-55 (100 or 400 nM). Then the cells were cultured in media containing transcription inhibitor actinomycin D (1 mg/ml) for 1, 2 and 4 h. The nNOS mRNA levels were determined at 1, 2 and 4 h of actinomycin D treatment and expressed as percentages of that immediately before actinomycin D treatment in each experimental group. Cells treated with actinomycin D only were used as a control (NC). *p<0.05 vs. control.