Skip to main content
. 2018 May 17;42(2):799–810. doi: 10.3892/ijmm.2018.3686

Figure 2.

Figure 2

HG treatment suppresses the expression of PRKAA1 and upregulates levels of miR-137 and IL-6. HTR-8/SVneo trophoblastic cells were treated with HG (25 mmol/l) medium for 24 h, with a control group cultured in normal medium (5 mmol/l). Expression levels of miR-137 and PRKAA1 were detected using reverse transcription-quantitative polymerase chain reaction analysis, with hsa-u6 and β-actin as internal controls. Protein levels of PRKAA1 (~62 kDa) and IL-6 were measured using western blot analysis and ELISA, respectively, and the relative density of PRKAA1 was determined using Image Pro Plus 6.0 software. (A) HG intervention suppressed the expression of PRKAA1 in HTR-8/SVneo cells. (B) miR-137 expression levels were elevated in HG-exposed HTR-8/SVneo cells. (C) Protein level of PRKAA1 was decreased in HG-exposed HTR-8/SVneo cells. (D) Results of western blot analysis. (E) HG treatment promoted the secretion of IL-6 in HTR-8/SVneo cells. Data are expressed as the mean ± standard error of the mean; statistical significance was determined using Student's t-test, *P<0.05, ***P<0.001. HG, high glucose; Ctrl, control; miR-137, microRNA-137; PRKAA1 protein kinase AMP-activated catalytic subunit α1; IL-6, interleukin-6.