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Cytotoxicity of icariin on HaCaT cells. The viability of HaCaT cells treated with 0-60 μM icariin for 24–72 h was analyzed with the CCK-8 assay. Data represent mean ± standard deviation of three independent experiments. Icariin concentration of 30 μM was regarded safe and used for the subsequent experiments. *P<0.05, **P<0.01 and ***P<0.001 compared with untreated control. CCK-8, cell counting kit-8; OD, optical density
