C57/Bl6 donor livers subjected to extended cold storage (18h) were transplanted to syngeneic mice. OLT recipients were treated i.v. with SER (5μg/kg) or equivalent amount of lactate ringer solution (control) at reperfusion. Liver and serum samples were collected at 6h post-surgery. (A) Representative H&E (upper panels, original magnification, ×100) and TUNEL (lower panels, original magnification, ×400) staining. (B) Suzuki's histological grading of liver IRI and quantification of TUNEL+ cells/HPF (n=5/group). (C) Serum ALT and AST levels (IU/L, n=5/group). Data shown as mean±SD (*p<0.05, Student t-test). (D) Animals were monitored for 14 days and cumultive survival was analyzed by Kaplan-Meier method. Solid line indicates SER-treated (n=13) while dotted line control (n=14) mice (*p<0.05, log-rank test).