Figure 6. Immunization with exosomes isolated from sera of rejected syngeneic graft induces rejection and immune responses to SAgs.

Exosomes containing the SAgs MYO and VIM were isolated from sera of rejected mice and exosomes from stable mice immediately following HTx. (A) Administration of exosomes from the sera of mice who experienced graft rejection induced cellular infiltration and myocyte damage to syngeneic heart grafts, resulting in rejection on day 7 (the controls did not experience rejection). (B) De novo development of Abs to SAgs in sera shows that exosomes containing SAgs induced Ab development to cardiac SAgs MYO and VIM in mice who experienced graft failure compared with control mice who did not experience graft failure. Five animals/group.