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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Cell Stem Cell. 2018 Jun 7;23(1):46–59.e5. doi: 10.1016/j.stem.2018.05.002

Figure 3. Radiation induced mature Paneth cells to proliferate and re-differentiate.

Figure 3

(A) Wild type mice were administered a single BrdU injection to label cycling cells. After a month, the tissue was analyzed.

(B-I) After one month, none of the remaining BrdU+ cells expressed markers of stem cell (C, G) or secretory precursor (D-F). All BrdU+ cells were positive for lysozyme (B) and surface UEA (H). Scale bar, 10 μm.

(J) One month after tamoxifen injection of Lyz1CreER/+; R26R-tdT mice, all remaining tdT+ cells were positive only for Paneth cell markers.

(K) Experimental scheme: one month after tamoxifen injection of Lyz1CreER/+; R26R-tdT mice, animals were irradiated then sacrificed 7 days after irradiation to determine whether radiation induces mature Paneth cell to proliferate.

(L, N) Radiated Lyz1CreER/+; R26R-tdT mice showed villus and crypt localized tdT+ stripes.

(M) Villus tdT+ cells in irradiated animals expressed enterocyte marker SI. Scale bar, 10 μm.

(O, P) Crypt tdT+ cells in irradiated animals showed PCNA+ (O) and Ki67+ (P) cells. Scale bar, 10 μm.