(A) Experimental scheme: Lyz1CreER;
R26R-NICD1-IRES-GFP mice were injected with tamoxifen, and
sacrificed at 8, 24, 48, 72, and 168 hours for tissue analyses. Mice were
labeled with EdU for 30 minutes before sacrifice.
(B-C) In non-tamoxifen treated mice, there was no
GFP+/NICD+ cell detected (0 hour).
After 8 hours GFP+ cells became readily detectable at crypt
bottom, and the majority of GFP+ cells were
EdU+. The number of
EdU+GFP+ cells expanded at 24 hours,
then reduced and disappeared from 48 hours onwards.
(D-E) Villus GFP+/NICD+ cells
were detected approximately 1 day after the initial detection of
GFP+ cells in the crypts. At 168 hours,
GFP+/NICD+ cell was no longer observed
suggesting a transient NICD-activated proliferation and differentiation. n.d.
not detected.
(F) Villus GFP+NICD+ cells
showed expression of enterocyte marker (SI) and goblet cell marker (Muc2). Scale
bar, 10 μm.