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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Cell Stem Cell. 2018 Jun 14;23(1):86–100.e6. doi: 10.1016/j.stem.2018.05.021

Figure 1. AML LSCs have higher expression of FIS1 and distinct mitochondrial morphology.

Figure 1

(A) A diagram showing regulation of healthy mitochondrial network through mito-fusion, mito-fission and mitophagy.

(B) qPCR results showing relative expression of FIS1 gene in sorted ROS-low LSCs (L) versus ROS-high non-LSCs (N). Mean ± SD (n=3). Type 2, two-tailed t-test.

(C) WB results showing expression of FIS1 protein in sorted ROS-low LSCs (L) versus ROS-high non-LSCs (N).

(D) Representative confocal images showing morphology of mitochondria in ROS-low LSCs versus ROS-high non-LSCs. Yellow arrows highlight distinct mitochondrial morphology.

(E) A diagram showing the method used to quantify the mitochondrial to nuclear area ratio.

(F) Mitochondrial to nuclear area ratio in LSCs vs. non-LSCs in 3 primary AML specimens. Each dot represents an individual cell. Mean ± SD. Type 3, two-tailed t-test.

(G) Representative TEM images showing morphology of mitochondria in ROS-low LSCs and ROS-high non-LSCs. Blue and red dotted lines outline mitochondrial shape.

(H) Quantification of mitochondrial cross-section area from the TEM images of AML 1. Each dot represents a single mitochondrion. Mean ± SD. Type 3, two-tailed t-test.

See also Figure S1.