KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-CD45-Alexa700 | BD | Cat# 560566, RRID:AB_1645452 |
| Anti-CD3-PECY7 | BD | Cat# 557749, RRID:AB_396855 |
| Anti-CD19-PE | BD | Cat# 555413, RRID:AB_395813 |
| Anti-Ki67-Alexa647 | Biolegend | Cat# 350510, RRID:AB_10900821 |
| Anti-H3K27ac | Active Motif | Cat# 39133, RRID:AB_2561016 |
| In vivo anti-human CD3 | BioXCell | Cat# BE0001-2, RRID:AB_1107632 |
| Rabbit anti-FIS1 | Proteintech | Cat# 10956-1-AP, RRID:AB_2102532 |
| Rabbit anti-DRP1 | Cell Signaling | Cat# 8570, RRID:AB_10950498 |
| Rabbit anti-AMPKa1 total | Cell Signaling | Cat# 2793, RRID:AB_915794 |
| Rabbit anti-AMPKa1-p-T172 | Cell Signaling | Cat# 2535, RRID:AB_331250 |
| Rabbit anti-AMPKa1/2 | Cell Signaling | Cat# 2532, RRID:AB_330331 |
| Rabbit anti-GSK3-α/β total | Cell Signaling | Cat# 5676, RRID:AB_10547140 |
| Rabbit anti-GSK3-α/β-p-Ser21/9 | Cell Signaling | Cat# 8566S, RRID:AB_10860069 |
| Rabbit anti-GSK3-β total | Cell Signaling | Cat# 12456, RRID:AB_2636978 |
| Rabbit anti-GSK3-β-p-Ser9 | Cell Signaling | Cat# 5558, RRID:AB_10013750 |
| Rabbit anti-PINK1 | Cell Signaling | Cat# 6946S, RRID:AB_11179069 |
| Rabbit anti-TBC1D15 | Sigma | Cat# HPA013388, RRID:AB_2199233 |
| Mouse anti-GAPDH | Santa Cruz | Cat# sc-32233, RRID:AB_627679 |
| Goat anti-ACTIN | Santa Cruz | Cat# sc-1616, RRID:AB_630836 |
| Biological Samples | ||
| Primary AML | Craig Jordan Lab | n/a |
| Normal CBMC | Craig Jordan Lab | n/a |
| Normal PBMC | Craig Jordan Lab | n/a |
| Chemicals, Peptides, and Recombinant Proteins | ||
| CellRox-DeepRed | Life Technologies | Cat# C10422 |
| MitoTracker-DeepRed FM | Life Technologies | Cat# M22426 |
| Polybrene (Hexadimethrine bromide) | Sigma | Cat# 107689-10G |
| Critical Commercial Assays | ||
| Mitochondria Isolation Kit for Cultured Cells | Thermo Scientific | Cat# 89874 |
| CD34 MicroBead Kit | Miltenyi Biotec | Cat# 130-046-702 |
| KWIK-DIFF Stain Kit | Thermo Scientific | Cat# 9990700 |
| ThruPLEX DNA-seq Kit | Rubicon Genomics | Cat# R400427 |
| Deposited Data | ||
| RNAseq of MOLM-13 cells with FIS1 knock-down | This paper | GSE114111 |
| RNAseq of primary AML with FIS1 knock-down | This paper | GSE114109 |
| RNAseq of primary AML with AMPK knock-down | This paper | GSE114105 |
| CHIPseq of MOLM-13 cells with FIS1 knock-down | This paper | GSE114106 |
| Experimental Models: Cell Lines | ||
| MOLM-13 | Tobias Neff Lab | n/a |
| 293T | Craig Jordan Lab | n/a |
| Experimental Models: Organisms/Strains | ||
| NSGS (NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3, CSF2,KITLG)1Eav/MloySzJ) | The Jackson Laboratory | Cat# JAX:013062, RRID:IMSR_JAX:013062 |
| Recombinant DNA | ||
| pLKO.1-GFP | Craig Jordan Lab | n/a |
| pLKO.1-Crimson | Craig Jordan Lab | n/a |
| pPax2 | Craig Jordan Lab | n/a |
| pMD2.G | Craig Jordan Lab | n/a |
| pLVX-EF1a-IRES-mCherry | Clontech | Cat# 631987 |
| LentiCRISPR-V2-GFP | Addgene | Cat# 82416 |
| HA GSK3 beta wt pcDNA3 | Addgene | Cat# 14753 |
| HA GSK3 beta S9A pcDNA3 | Addgene | Cat# 14754 |
| Software and Algorithms | ||
| bcl2fastq v1.8.4 | Illumina | n/a |
| Trimmomatic v0.32 | Usadel Lab | n/a |
| cufflinks v2.0.2 | Trapnell Lab | n/a |
| Bowtie2 v2.2.6 | Salzberg Lab | n/a |
| Flowjo v10.1 | Flowjo | n/a |
| Prism v6.0h | Prism | n/a |
| ImageJ v1.51s | Imagej.nih.gov | n/a |