Figure 5.
Effects of oviposition and feeding on expression of N. attenuata genes. Transcript accumulation (mean ± SEM, N = 9–10) of (a) PTR1 homolog (oligo peptide transporter: Na_454_15271), (b) WRKY transcription factor (WRKY 71-like: Na_454_21846), (c) a defensin-like protein (Na_454_03111), (d) L-aspartate oxidase (AO: Na_454_00281), (e) trypsin protease inhibitor NaPI (Na_454_02256) and (f) geranylgeranyl diphosphate synthase NaGGPPS2 (Na_454_01146) in N. attenuata plants in response to the oviposition by and later the feeding larvae of either S. exigua or M. sexta. Transcript accumulation relative to untreated plants was determined by real-time qPCR normalized to β-actin and EF1α as reference genes. Asterisks indicate significant differences at P < 0.05/0.005/0.001 according to linear mixed-effects models with oviposition and larvae of either S. exigua or M. sexta as fixed factors and replicate blocks as random factor.