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. 2018 Jul 6;8:10249. doi: 10.1038/s41598-018-28233-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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N. attenuata signalling genes differentially induced by larvae of M. sexta and S. exigua. Transcript accumulation (mean ± SEM) of selected genes known to be key regulators in different plant signalling pathways: (a) threonine deaminase (NaTD: Na_454_03285), (b) aminocyclo-propanecarboxylate oxidase (NaACO2: Na_454_06634), (c) transcription factor WRKY6 (Na_454_14052), (d) SA receptor (NaNPR1: Na_454_12995) in plants fed by either S. exigua (Se, dark bars) or M. sexta (Ms, light bars) larvae. The plants were previously oviposited by either of the two species or not (−). Transcript accumulation relative to untreated plants was determined by real-time qPCR and normalized to β-actin and EF1α as reference genes. Letters indicate significant differences at P < 0.05 according to linear mixed-effects models with the different herbivory treatments as fixed factors and replicate blocks as random factor followed by a Tukey post-hoc test (N = 9–10).