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. 2018 Jul 6;8:10251. doi: 10.1038/s41598-018-27602-y

Figure 3.

Figure 3

GAG production in pellets. (A) Alcian blue staining, nuclei were counterstained by nuclear fast red; (B) Safranin O staining, nuclei were counterstained by haematoxylin; (C) GAG and DNA assay. N, normoxia; H, hypoxia; *p < 0.05; **p < 0.01; #represents a significant difference between normoxia and hypoxia (p < 0.05).