Fig. 5.

IMD suppressed the recruitment of macrophages from bone marrow to the periphery via decreasing CCR2^high cells. The bone marrow samples (a, d, g) and blood samples (j, m, p) from WT, IMD^−/−, or IMD^−/− mice rescued by IMD₄₀ injection were analyzed using flow cytometry. Gating strategy: monocyte progenitors (MoP, identified as Ly6C^high/CX3CR⁺); monocytes (Mo, identified as CD115⁺/CD11b⁺); macrophages (Mϕ, identified as F4-80⁺/CD11b⁺). Ratio of CCR2^high (%) in BMMoP (b), BMMo (e), BMMϕ (h), blood MoP (k), blood Mo (n), and blood Mϕ (q) were quantified. The chemotaxis toward MCP-1 (CCL2) of the BMMoP (c), BMMo (f), BMMϕ (i), blood MoP (l), blood Mo (o), and blood Mϕ (r) were tested and quantified. Significance was assessed by Kruskal–Wallis test followed by non-parametric Dunn’s post-hoc analysis