Skip to main content
. 2018 Jul 6;50(7):1–9. doi: 10.1038/s12276-018-0114-1

Fig. 1. NE increases IL-8 production via PAR2-mediated activation of ERK.

Fig. 1

a BEAS-2B cells were treated with vehicle control (VC) or NE (1 U/ml) for the indicated times. Total cellular extracts were subjected to western blot analysis for p-ERK and GAPDH. b Cells were pre-treated with an MEK inhibitor (U0126, 20 μM) for 1 h and then stimulated with VC or NE (1 U/ml) for 24 h. Levels of IL-8 in cell supernatants were measured by multiplex bead assay. Data represent the mean ± SD; **P < 0.05. c–e BEAS-2B cells were transiently transfected with control siRNAs or PAR2 siRNAs using a Neon electroporation kit. Forty-eight hours after transfection, cells were stimulated with VC or NE for 1, 2, 4 h (d) or 24 h (e). The expression of PAR2 was measured by quantitative real-time PCR. Data were normalized to the expression of GAPDH. Data represent the mean ± SD; **P < 0.05. Total cellular extracts were subjected to western blot analysis for PAR2, p-ERK and GAPDH. IL-8 concentrations in culture media were measured by multiplex bead assay. Data represent the mean ± SD; **P < 0.05