Fig. 5.

EEA-motif targeting improves NANOG and REX1 activation. a Immunostaining of emerging iPSC colonies detects activation of targeted LIN28A before its mRNA reads become detectable in bulk RNA-seq. Scale bar = 400 µm. b HFF reprogramming efficiency after transgenic expression of additional factors belonging to Group 6 (Fig. 4c). n = 5, three independent experiments. c qRT-PCR quantification of NANOG and REX1 activation using dCas9VP192 with and without EEA-motif gRNAs in HEK293. n = 3, three independent experiments. d NANOG activation can be detected by immunostaining in CRISPRa reprogramming colonies 2 days earlier in the presence of EEA-motif targeting guides. Scale bar = 200 µm. Data presented as mean ± s.e.m., two tailed Student’s t-test. **P < 0.01, ***P < 0.001