Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 6;8:10241. doi: 10.1038/s41598-018-28625-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Direct interaction between EhMIF and EhJAB1. (A) Schematic of GST pull-down assay. (B) Purified recombinant GST and GST-EhJAB1 proteins (arrowhead) used in the GST pull-down assay were separated by SDS/PAGE and stained with Coomassie Blue. (C) Interaction between EhMIF and EhJAB1 by GST pull-down assay. GST-EhJAB1 and GST control were mixed with EhMIF. Input (10%) and pull-down material were separated by SDS/PAGE, and EhMIF was detected by immunoblot analysis using anti-EhMIF antibody. (D) Schematic diagram of co-immunoprecipitation assay. (E,F) Co-immunoprecipitation of EhMIF and biotinylated EhJAB1 (EhJAB1^B) mixture using specific anti-EhMIF antibody bound beads. EhJAB1^B was detected by immunoblot analysis using goat anti-biotin HRP conjugated antibody.