Skip to main content
. 2018 Jul 6;8:10241. doi: 10.1038/s41598-018-28625-1

Figure 3.

Figure 3

Characterization of EhMIF binding to EhJAB1 using the Biolayer interferometry. (A) Representative binding and dissociation curves for EhMIF binding to EhJAB1. Anti-GST antibody-coated biosensors were loaded with GST-EhJAB1. Sensors were placed into solutions with EhMIF, concentrations range from 5 to 80 nM (association analysis: 0 to 600 secs). Subsequently, the sensors were transferred to buffer without EhMIF for dissociation analysis (from 600 to 1200 secs). Analysis revealed a dissociation constant of KD of 3.86 × 10−8 M. (B) Biosensors loaded with GST only were used as controls.