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. 2018 Jul 6;8:10241. doi: 10.1038/s41598-018-28625-1

Figure 4.

Figure 4

Mapping the EhMIF-interacting domain of EhJAB1. (A) Predicted interaction between EhMIF and EhJAB1. EhMIF (red) and EhJAB1 N-terminal (blue), MPN domain (yellow), JAMM motif (purple) and C-terminal (green). (B) Schematic representations of full-length and deletion mutants of EhJAB1. Δ 1 (deletion of 3–50 aa), Δ 2 (deletion of 51–133 aa), Δ 3 (deletion of 134–147 aa), Δ 4 (deletion of 148–187 aa), Δ 5 (deletion of 187–318 aa), Δ 6 (deletion of 247–318 aa). (C) PCR amplicons and protein expression of GST-tagged full-length EhJAB1 and GST-tagged deletion mutants of EhJAB1. (D) Interactions of EhMIF with deletion mutants were analyzed by immunoblotting.