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. 2018 Jul 6;8:10241. doi: 10.1038/s41598-018-28625-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of EhJAB1 binding on EhMIF activity. (A) EhJAB1 inhibits EhMIF interaction with the human MIF receptor CD74 by GST pull-down competition assay. Input (lane 1) and GST control (lane 2). EhMIF was preincubated with or without EhJAB1 at increasing doses before mixing with GST-CD74 (lanes 3–6). (B,C) EhJAB1 inhibits EhMIF-induced IL-8 production in a dose-dependent manner. Human colonic epithelial cells HCT116 and Caco2 were incubated with EhMIF with or without EhJAB1 at increasing doses. Culture supernatants were collected after 8 h and IL-8 was quantified by ELISA. Data represent mean and SD of triplicates from 1 experiment and are representative of 3 independent experiments. *P < 0.01; **P < 0.001. ns, not significant.