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. 2018 Jul 6;8:10283. doi: 10.1038/s41598-018-28486-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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α-SMA immunohistochemistry in liver. (A–D) α-SMA immunostaining (brown) and haematoxylin counterstain in control (A), DEN/TAA (B) and DEN/TAA/HFD (C,D) treatment groups. (E–L) Examples of the α-SMA expression in different types of lesions observed in DEN/TAA/HFD treated mice. Boxes formed by black dashes indicate higher magnification of adjacent images. (M) Quantitation of α-SMA immunopositivity by area in the whole liver section. Representative data from 5–6 mice per group. Statistical analysis used Kruskal-Wallis test, *p < 0.05, **p < 0.01. Scale Bars = 400 μm (J–L), 500 μm (A–C), and 1000 μm (I).