Fig. 7.

Summary of mutations at the glycan binding site in the prevalent strains. a The schematic diagram of glycan recognition in VP8*s of P[6], P[4], P[8], and P[19] genotypes that belong to the P[II] genogroup shows the common residues (black) interact with the Fuc-Gal-GlcNAc moieties, and the sequence changes (blue) lead to distinct recognition of the reducing end of the glycan in VP8*s. The molecular interactions between H type I glycan and P[4]/neonatal P[6] VP8*s are indicated by blue, red and black lines. The corresponding residues in P[6], P[8], and P[19] VP8*s are shown in the lateral columns. b Sequence alignment of VP8*s of the P[II] genogroup including those of porcine rotavirus (PRV) P[6], neonatal and non-neonatal P[6] HRV¹⁶. The conserved residues that recognize the type I precursor are indicated black shades, and other glycan binding residues are indicated by blue shade. The mutations at residue 169 are highlighted with green and red shades