Figure 5.

The conditioned medium of NE-like cells modifies the invasive capacity of PC3 cells through GRPR. (a) Invasion assay was performed by using a Boyden chamber. PC3 cells (6 × 10⁴) suspended in 200 µl of culture medium without FBS were placed in the upper chamber. 700 µl of conditioned medium from 96 h Control siRNA and 96 h AR siRNA treated with or without baclofen (100 µM) was added in the lower chamber for 6 h. Results are represented as mean ± SEM of three independent experiments in triplicate wells, *p < 0.05, **p < 0.001 and ***p < 0.0001. (b) GRPR mRNA expression was analyzed by RT-qPCR. Results are presented as mean ± SEM of three independent experiments in triplicate, *p < 0.05 **p < 0.001 and ***p < 0.0001. GRP synthetized was used as positive control; the previous concentration represents 35% of conditioned medium from 96 h AR siRNA treated with baclofen (100 µM) that was used to perform the experiments.