Fig. 5.

Nod2^−/− mice show reduced histological damage and fungal burden following Aspergillus infection. a Histology of lung sections of wild-type and Nod2^−/− mice at day 3 pi, stained in HE (I, II), Grocott’s Methenamine Silver (III) or labelled using anti-F4/80 antibody (specific for macrophages), counterstained with Haematoxylin staining. Scale bars represent 1 mm (I) and 200 μm (II–IV). b Morphometric analysis of the lesions in the whole lung sections using Image J software to quantify the lesions in number and size. c Representative lung sections of two additional WT and Nod2^−/− mice, stained in HE (I) and Grocott’s Methenamine Silver (II–IV). Scale bars represent 200 μm (I, II) and 50 μm (III, IV), means were compared for significance using the Mann-Whitney U test