Fig. 9.
MDP negatively affects antifungal host response only in the presence of functional NOD2. a, b The fungal killing capacity of (a) murine BMDMs (n = 8 wt and n = 6 Nod2−/−) and (b) human monocytes (1 × 105) assessed by CFU remaining of A. fumigatus (2 × 106) following exposure for 24 h. c Phagocytosis efficiency assessed as percentage of monocytes that engulfed FITC-labelled A. fumigatus conidia and mean fluorescence intensity of the total macrophage population, (d) surface dectin-1 expression measured by flow cytometry in cells of healthy controls (co) (n = 6) as well as NOD2-deficient patients (NOD2−/− PT) (n = 3). Data are represented as scatterplot with median, black dots represent wild-type and grey dots represent NOD2-deficient cells. Means were compared using the Wilcoxon signed-rank test for paired comparisons except for the comparison of WT with Nod2-/- cells which were compared using the Mann-Whitney U test