Fig. 1.

P. aeruginosa pmrB mutants are resistant to host defence molecules and lysozyme killing. Supernatants from phorbol 12-myristate 13-acetate (PMA)-stimulated (a) HL-60 or (b) mouse bone marrow neutrophil cultures were transferred to mid-log growth P. aeruginosa. Colony forming units (CFU) as a percentage of control. Control bacteria were exposed to supernatant from non-activated (no PMA) HL-60 cultures. c Optical density at 600 nm of P. aeruginosa grown in the presence of lysozyme (mean ± s.d.). Results are expressed as a percentage of the no-lysozyme control for each isolate and are a composite of 3 biological replicates per isolate, each containing 3 technical replicates. Asterisks (*) represent significant differences in ANOVA performed on CFU counts (a, b) or OD readings, with lysozyme as a covariant (c), with Dunnett’s multiple comparison test vs. LESB65. *p < 0.05, **p < 0.01, ***p < 0.005